Search results for "Fibroblast-like stem cells"

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Donor age and long-term culture do not negatively influence the stem potential of limbal fibroblast-like stem cells

2016

AbstractBackgroundIn regenerative medicine the maintenance of stem cell properties is of crucial importance. Ageing is considered a cause of reduced stemness capability. The limbus is a stem niche of easy access and harbors two stem cell populations: epithelial stem cells and fibroblast-like stem cells. Our aim was to investigate whether donor age and/or long-term culture have any influence on stem cell marker expression and the profiles in the fibroblast-like stem cell population.MethodsFibroblast-like stem cells were isolated and digested from 25 limbus samples of normal human corneo-scleral rings and long-term cultures were obtained. SSEA4 expression and sphere-forming capability were ev…

0301 basic medicineHomeobox protein NANOGCellular differentiationMedicine (miscellaneous)BiologyStem cell markerBiochemistry Genetics and Molecular Biology (miscellaneous)Settore MED/13 - Endocrinologia03 medical and health sciencesAdult stem cell pluripotency; Fibroblast-like stem cells; Limbal stem cells; Proteomic profile; Regenerative medicineLimbal stem cellStem cell transplantation for articular cartilage repairAdult stem cell pluripotencyInduced stem cellsResearchFibroblast-like stem cellProteomic profileCell BiologyCell biologyEndothelial stem cell030104 developmental biologyRegenerative medicineMolecular MedicineLimbal stem cellsStem cellFibroblast-like stem cellsAdult stem cellStem Cell Research & Therapy
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In Vitro Generation of Pancreatic Endocrine Cells from Human Adult Fibroblast-Like Limbal Stem Cells

2012

Stem cells might provide unlimited supply of transplantable cells for β-cell replacement therapy in diabetes. The human limbus is a highly specialized region hosting a well-recognized population of epithelial stem cells, which sustain the continuous renewal of the cornea, and the recently identified stromal fibroblast-like stem cells (f-LSCs), with apparent broader plasticity. However, the lack of specific molecular markers for the identification of the multipotent limbal subpopulation has so far limited the investigation of their differentiation potential. In this study we show that the human limbus contains uncommitted cells that could be potentially harnessed for the treatment of diabete…

Pluripotent Stem CellsStromal cellCellular differentiationPopulationBiomedical Engineeringlcsh:MedicineEnteroendocrine cellLimbus CorneaeBiologyLimbus; β-CellsSettore MED/13 - EndocrinologiaLimbuInsulin-Secreting CellsInsulin SecretionDiabetes MellitusHumansInsulineducationInduced pluripotent stem cellCells CulturedProinsulinTransplantationeducation.field_of_studyDiabeteslcsh:RCell DifferentiationCell Biologyβ-CellsCell biologyAdult Stem CellsStem cellFibroblast-like stem cellsBiomarkersAdult stem cellCell Transplantation
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